ADC target antigens in solid tumors

Antibody–drug conjugates (ADCs) are exciting new anticancer therapeutics. ADCs include target-specific monoclonal antibodies (mAbs), cytotoxic drugs, and chemically synthesized linkers that covalently bind them.

ADCs are a rapidly growing drug therapy for solid tumors due to the cytotoxic effects of chemotherapeutic drugs combined with antibodies targeting tumor-associated antigens (TAAs).

The ADCs mAb attaches to specific antigens on the surface of tumor cells, and ADCs are internalized into tumor cells during the formation of the antibody–antigen complex. ADCs are typically moved from the endosome to the lysosome, where the linker is divided and small molecule cytotoxic drugs are released, resulting in tumor cell death.

In addition to the long-standing ADC target antigens, such as FGFR, EGFR, c-MET, and HER2, new targets like EpCAM, TROP-2, CD25, and others (Table 1) are being researched and show promise in the treatment of solid tumors.

Sino Biological is at the forefront of advancing ADC therapy for treating solid tumors by providing high-quality ADC target antigens.

Table 1. ADC target antigens at the preclinical and clinical stages. Source: Sino Biological Inc.

Various categories of ADCs in clinical and preclinical stages ADC target antigens
Well-established ADC TAAs HER2, EGFR, EGFRvIII, c-MET, FGFR2, FGFR3
ADC TAAs overexpressed on cancer cells EpCAM, BCMA, TROP-2, AXL, HER3, CD166, CEACAM5, GPNMB, Mesothelin, CD70
Target antigens in TME CD25/IL2R, B7-H3, ANTXR1
Target antigens in CSCs PTK7, ROR1, 5T4

 

ADC target antigens in solid tumors

Figure 1. General mechanism of action of ADCs. Image Credit: Sino Biological Inc.

Featured recombinant proteins

Sino Biological offers superior products that have been substantiated for activity and are highly regarded by users, as evidenced by citations in top-tier journals.

  • High-purity
  • High-activity
  • HPLC-verified
  • Cited by researchers

Human EGFR protein Cat#: 10001-H27H-B

ADC target antigens in solid tumors

Immobilized Human EGF (Cat#: 10605-H01H) can bind Human EGFR. Image Credit: Sino Biological Inc.

Human c-MET protein Cat#: 10692-H08H

ADC target antigens in solid tumors

Immobilized Cynomolgus HGF (Cat#: 90286-CNAH) can bind Human c-MET. Image Credit: Sino Biological Inc.

Human CD25/IL2R alpha protein Cat#: 10165-H08H

ADC target antigens in solid tumors

Ability to inhibit IL2-induced proliferation of M07e cells. Image Credit: Sino Biological Inc.

Human BCMA protein Cat#: 10620-H08H

ADC target antigens in solid tumors

Immobilized Human BAFF (Cat#: 10056-H01H) can bind Human BCMA. Image Credit: Sino Biological Inc.

Human CD70 protein Cat#: 10780-H01H

ADC target antigens in solid tumors

Immobilized Human CD27 (Cat#: 10039-H08B1) can bind Human CD70. Image Credit: Sino Biological Inc.

Human CD166/ALCAM protein Cat#: 10045-H08H

ADC target antigens in solid tumors

Immobilized human CD166 can bind mouse CD6. Image Credit: Sino Biological Inc.

More recombinant proteins (Partial)

Table 2. Source: Sino Biological Inc.

Cat# Molecule Species Expression Host Purity Activity
10001-H08H EGFR Human HEK293 Cells > 90 % Active
51091-M08H Mouse HEK293 Cells > 85 % Active
80100-R08H Rat HEK293 Cells > 98 % Active
90317-K02H Rhesus HEK293 Cells > 95 %  
90285-C08H Cynomolgus HEK293 Cells > 90 % Active
10692-H27H-B c-MET Human HEK293 Cells > 95 % Active
50622-M08H Mouse HEK293 Cells > 90% Active
80004-R02H Rat HEK293 Cells > 95 % Active
90304-C08H-B Cynomolgus, Rhesus HEK293 Cells > 90 %  
10694-H08H EpCAM Human HEK293 Cells ≥ 90 % Active
50591-M08H Mouse HEK293 Cells > 95 %  
80306-R08H Rat HEK293 Cells > 97 %  
10428-H03H TROP2 Human HEK293 Cells > 98 % Active
50922-M02H Mouse HEK293 Cells > 95 % Active
86246-R08H Rat HEK293 Cells > 90 %  
10165-H02H CD25/IL2Rα Human HEK293 Cells > 90 % Active
50292-M02H Mouse HEK293 Cells > 95 % Active
80074-R08B Rat Baculovirus-Insect Cells > 95 %  
90265-C02H Cynomolgus HEK293 Cells > 90 % Active
10620-H40H-B BCMA Human HEK293 Cells > 95 % Active
50076-M42H-B Mouse HEK293 Cells > 90 % Active
90103-C35H-B Rhesus HEK293 Cells > 95 % Active
10824-H03H FGFR2 Human HEK293 Cells > 90 % Active
51128-M02H Mouse HEK293 Cells > 95 % Active
16486-H02H FGFR3 Human HEK293 Cells > 95 % Active
50071-M08H Mouse HEK293 Cells > 98 % Active
90313-C02H Cynomolgus HEK293 Cells > 90 % Active
50005-M03H CD166/ALCAM Mouse HEK293 Cells > 95 % Active
80221-R02H Rat HEK293 Cells > 95 % Active
90227-C08H Cynomolgus HEK293 Cells > 95 % Active
51129-M04H CD70 Mouse HEK293 Cells > 95 % Active
80161-R01H Rat HEK293 Cells > 95 % Active
10279-H08H AXL Human HEK293 Cells > 90 %  
50126-M08H Mouse HEK293 Cells > 98 %  
83136-R08H Rat HEK293 Cells > 95 %  
10201-H02H HER3 Human HEK293 Cells > 90 % Active
51003-M08H Mouse HEK293 Cells > 93 % Active
80111-R08H Rat HEK293 Cells > 95 % Active
11077-H27H-B CEACAM5 Human HEK293 Cells > 95 %  
54867-M02H1 Mouse HEK293 Cells > 90 %  
11305-H02H GPNMB Human HEK293 Cells > 95 %  
13128-H08H Mesothelin Human HEK293 Cells > 90 %  
11188-H02H B7-H3 Human HEK293 Cells > 95 %  
50973-M02H Mouse HEK293 Cells > 95 %  
13367-H02H ANTXR1 Human HEK293 Cells > 90 %  
19399-H08H CCK4/PTK7 Human HEK293 Cells > 95 %  
13968-H20B ROR1 Human Baculovirus-Insect Cells > 85 % Active
13968-H02H1 Human HEK293 Cells > 90 %  
19845-H08H 5T4/TPBG Human HEK293 Cells > 95 %  

 

Other related proteins

  • TIM-1
  • CD56
  • EpCAM
  • Siglec-2
  • Ly75
  • CD228
  • Fra
  • Nectin-4
  • MUC1
  • CD71
  • ENPP3
  • Liv1

Antibodies for ADC target antigens

Sino Biological has created a panel of antibodies for ADC target antigens that can be used in ELISA, IHC, IP, FCM, and WB, among other applications.

Anti-EpCAM antibody rabbit MAb Cat#: 50591-R002

ADC target antigens in solid tumors

Immunochemical staining of mouse EpCAM in mouse stomach. Image Credit: Sino Biological Inc.

Anti-EGFR antibody rabbit MAb Cat#: 10001-R021

ADC target antigens in solid tumors

Immunofluorescence staining of Human EGFR in A431 cells. Image Credit: Sino Biological Inc.

Anti-c-MET antibody rabbit MAb Cat#: 10692-R271-A

ADC target antigens in solid tumors

Profile of anti-human C-Met reactivity on HepG2 cells analyzed by flow cytometry. Image Credit: Sino Biological Inc.

More antibodies for ADC target antigens (Partial)

Table 3. Source: Sino Biological Inc.

Cat# Antigen Species Clonality Ig Type Application
10001-R021 EGFR Human Rabbit MAb Rabbit IgG ELISA, ICC/IF
10001-MM01   Human Mouse MAb Mouse IgG2b ELISA
10692-R243-F c-MET Human Rabbit MAb Rabbit IgG FCM
10692-MM02   Human Mouse MAb Mouse IgG1 ELISA
10694-R028 EpCAM Human Rabbit MAb Rabbit IgG WB, ELISA, IHC-P, FCM, ICC/IF, IP
10694-MM05   Human Mouse MAb Mouse IgG1 WB
10428-R001 TROP2 Human Rabbit MAb Rabbit IgG ELISA, ICC/IF, FCM
10428-MM01   Human Mouse MAb Mouse IgG1 ELISA, IHC-P, FCM
50292-R049-C CD25/IL2Rα Mouse Rabbit MAb Rabbit IgG FCM
10165-MM17-F   Human Mouse MAb Mouse IgG1 FCM
10620-R049 BCMA Human Rabbit MAb Rabbit IgG ELISA
10824-R016 FGFR2 Human Rabbit MAb Rabbit IgG ELISA
50071-R046 FGFR3 Mouse Rabbit MAb Rabbit IgG ELISA
100550-MM07   Human Mouse MAb Mouse IgG1 IHC-P
50005-R311 CD166/ALCAM Mouse Rabbit MAb Rabbit IgG ELISA, FCM
10045-MM05   Human Mouse MAb Mouse IgG1 ELISA
10279-R101-F AXL Human Rabbit MAb Rabbit IgG FCM
10279-MM17   Human Mouse MAb Mouse IgG1 ELISA
10201-R016 HER3 Human Rabbit MAb Rabbit IgG ELISA
10201-MM01   Human Mouse MAb Mouse IgG1 ELISA
11077-R327 CEACAM5 Human Rabbit MAb Rabbit IgG IHC-P
11077-MM08   Human Mouse MAb Mouse IgG2a ELISA, IHC-P
11305-R105 GPNMB Human Rabbit MAb Rabbit IgG ELISA
13128-MM12 Mesothelin Human Mouse MAb Mouse IgG1 ELISA, IHC-P
11188-MM06 B7-H3 Human Mouse MAb Mouse IgG2b ELISA, ICC/IF, FCM
50973-R317   Mouse Rabbit MAb Rabbit IgG ELISA
204330-T08 CCK4/PTK7 Human Rabbit PAb Rabbit IgG IHC-P
13968-T16 ROR1 Human Rabbit PAb Rabbit IgG ELISA
101430-T08 5T4/TPBG Human Rabbit PAb Rabbit IgG IHC-P

 

References

  1. Tang H, Liu Y, Yu Z, et al. (2019) The analysis of key factors related to ADCs structural design[J]. Frontiers in Pharmacology10, p. 373.
  2. Cai X, Yu J, Fang T, et al. (2022) Antibody-Drug Conjugates Targeting the Human Epidermal Growth Factor Receptor (HER) Family in Cancers[J]. Frontiers in Molecular Biosciences, 184.