FCγ receptors (FCγRII/CD32, FCγRIII/CD16, and FCγRI/CD64) are known as cellular receptors for IgG that add up to immune complex clearance, antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and comprise several subtypes.

FCγR polymorphisms can potentially forecast responses to immunotherapies, thereby directing personalized treatment methods. As far as therapeutic antibody development is concerned, the efficacy of mAbs is identified by their affinity to FCγRs, which impacts their engagement with immune effector cells.

FcγR-binding is vital for developing immune checkpoint antibodies against CTLA-4, PD-1, TIM-3, PD-L1, LAG3, CD73, TIGIT, VISTA, and B7-H3. Interactions of mAb with FcγRs also affect cytokine release, which is vital in the immune response. Moreover, FCγRs can serve as biomarkers to monitor treatment responses and are studied in combination therapies such as HER2-targeted mAb therapy. 

As a leading bioreagents company, Sino Biological offers high-purity and bioactivity-validated FcγR and other-related products to assist the development of new antibody therapies.

Table 1. The types and characteristics of FcγRs. Source: Sino Biological US Inc

Name FcγRI/CD64 FcγRIIA/CD32a FcγRIIB/CD32b FcγRIIC/CD32c FcγRIIIA/CD16a   FcγRIIIB/CD16b
Structure
Function Activating Activating Inhibitory Activating Activating Activating
Affinity High Low Low Low Low Low
Effect of Antibody Binding ADCP, cytokine release ADCC, ADCP, vaccinal effect Inhibits ADCC, ADCP, B cell activation Enhances ADCC, ADCP, B cell activation ADCC, ADCP Decoy receptor that inhibits ADCP

 

Featured recombinant FcγR proteins

Sino Biological has developed a panel of high-quality FcγR proteins from several species to assist in developing antibody therapies.

  • High-Purity
  • Activity-Validated
  • HPLC-Verified
  • Cited by scientists
  • Validated by BLI Assay

Human CD16a protein (His & AVI Tag)

(Cat#: 10389-H27H1-B)

BLI assay: Loaded biotinylated human CD16a protein on SA Biosensor can bind Bevacizumab (IgG1).

BLI assay: Loaded biotinylated human CD16a protein on SA Biosensor can bind Bevacizumab (IgG1). Image Credit: Sino Biological US Inc

ELISA functional assay: Immobilized biotinylated human CD16a protein can bind human IgG1 (Cat#: 10702-HNAC). Human CD32a Protein (His & AVI Tag) (Cat#: 10374-H27H-B) HPLC-verified 9 Citations.

ELISA functional assay: Immobilized biotinylated human CD16a protein can bind human IgG1 (Cat#: 10702-HNAC). Human CD32a Protein (His & AVI Tag) (Cat#: 10374-H27H-B) HPLC-verified 9 Citations. Image Credit: Sino Biological US Inc

Human CD32a protein (His & AVI Tag)

(Cat#: 10374-H27H-B)

ELISA functional assay: Immobilized human IgG1 at can bind human biotinylated CD32a protein.

ELISA functional assay: Immobilized human IgG1 at can bind human biotinylated CD32a protein. Image Credit: Sino Biological US Inc

Human CD32b protein (His & AVI tag)

(Cat#: 10259-H27H-B)

BLI assay: Loaded biotinylated human CD32b/c protein on SA Biosensor can bind Bevacizumab (IgG1).

BLI assay: Loaded biotinylated human CD32b/c protein on SA Biosensor can bind Bevacizumab (IgG1). Image Credit: Sino Biological US Inc

Human CD64 protein (His & AVI tag)

(Cat#: 10256-H27H-B)

ELISA functional assay: Immobilized Rituximab (IgG1) can bind biotinylated human CD64 protein.

ELISA functional assay: Immobilized Rituximab (IgG1) can bind biotinylated human CD64 protein. Image Credit: Sino Biological US Inc

Mouse CD64 protein (His & AVI tag)

(Cat#: 50086-M27H-B)

ELISA functional assay: Immobilized Rituximab (IgG1) can bind biotinylated mouse CD64 protein.

ELISA functional assay: Immobilized Rituximab (IgG1) can bind biotinylated mouse CD64 protein. Image Credit: Sino Biological US Inc

More recombinant FcγR proteins (Partial)

Table 2. Source: Sino Biological US Inc

Cat# Proteins Species Expression Host Purity Activity Tag
10389-H08C1
    8 Citations
CD16a Human CHO Cells ≥ 95 % (SEC-HPLC) Active C-His
10389-H08H1
    3 Citations
CD16a Human HEK293 Cells > 95 % (SEC-HPLC) Active C-His
10389-H27H-B
    10 Citations
CD16a Human HEK293 Cells > 95 % (SEC-HPLC) Active C-AVI & His
10389-H27H1
    3 Citations
CD16a Human HEK293 Cells > 90 % Active C-AVI & His
80017-R08H
    1 Citation
CD16a Rat HEK293 Cells > 97 % Active C-His
90013-C08H1-B CD16a Cynomolgus HEK293 Cells > 90 %   C-His
11046-H08H1 CD16b Human HEK293 Cells > 90 % (SEC-HPLC) Active C-His
11046-H27H-B
    1 Citation
CD16b Human HEK293 Cells > 95 % (SEC-HPLC) Active C-AVI & His
11046-HNAE-B CD16b Human E. coli > 90 % Active Native
50326-M08H
    5 Citations
CD16 Mouse HEK293 Cells > 95 % Active C-His
50326-M27H-B
    3 Citations
CD16 Mouse HEK293 Cells > 95 % Active C-AVI & His
90013-C27H-B
    1 Citation
CD16 Rhesus HEK293 Cells > 92 % Active C-AVI & His
10374-H08C1 CD32a Human CHO Cells ≥ 90 % (SEC-HPLC) Active C-His
10374-H08H1
    2 Citations
CD32a Human HEK293 Cells > 90 % (SEC-HPLC) Active C-His
10374-H27H2-B CD32a Human HEK293 Cells > 95 % (SEC-HPLC) Active C-AVI & His
80015-R08H CD32a Rat HEK293 Cells > 95 %   C-His
90015-C27H-B CD32a Cynomolgus HEK293 Cells > 95 % Active C-AVI & His
90016-K49H-B CD32a Rhesus HEK293 Cells ≥ 95 % (SEC-HPLC)   C-AVI & His
10259-H08C
    1 Citation
CD32b Human CHO Cells > 90 % (SEC-HPLC) Active C-His
10259-H08H
    5 Citations
CD32b Human HEK293 Cells > 95 % Active C-His
50030-M08H
    1 Citation
CD32b Mouse HEK293 Cells > 90 % (SEC-HPLC) Active C-His
80018-R08H CD32b Rat HEK293 Cells > 97 % Active C-His
90014-C27H-B CD32b Cynomolgus HEK293 Cells > 97 % Active C-AVI & His
10256-H08H
    11 Citations
CD64 Human HEK293 Cells > 90 % (SEC-HPLC) Active C-His
10256-H08S
    2 Citations
CD64 Human CHO Stable Cells > 95 % Active C-His
50086-M08H
    2 Citations
CD64 Mouse HEK293 Cells > 90 % (SEC-HPLC) Active C-His
80016-R08H
    1 Citation
CD64 Rat HEK293 Cells > 95 % Active C-His

 

Featured antibodies for FcγRs

A panel of antibodies for FcγRs and also related molecules with applications in IHC-P, FCM, ELISA, and WB has been developed by Sino Biological.

Anti-CD32a, mouse mAb

(Cat#: 10374-MM02-F)

Flow cytometric analysis of human CD32a expression on human whole blood lymphocytes (left panel), monocytes (middle panel), and granulocytes (right panel).

Flow cytometric analysis of human CD32a expression on human whole blood lymphocytes (left panel), monocytes (middle panel), and granulocytes (right panel). Image Credit: Sino Biological US Inc

Anti-CD16a, rabbit pAb

(Cat#: 10389-T24)

Applications: ELISA, IHC-P

Immunochemical staining CD16a in human spleen with rabbit polyclonal antibody.

Immunochemical staining CD16a in human spleen with rabbit polyclonal antibody. Image Credit: Sino Biological US Inc

Anti-CD32b, rabbit mAb

(Cat#: 10259-R110)

Applications: WB, IHC-P

Daudi Whole Cell (A) and THP1 Whole Cell (B) Lysate probed with anti-CD32b rabbit monoclonal antibody.

Daudi Whole Cell (A) and THP1 Whole Cell (B) Lysate probed with anti-CD32b rabbit monoclonal antibody. Image Credit: Sino Biological US Inc

Anti-CD64, rabbit mAb

(Cat#: 50086-R027)

Applications: FCM, ICC/IF

Immunochemical staining CD16a in human spleen with rabbit polyclonal antibody.

Immunochemical staining CD16a in human spleen with rabbit polyclonal antibody. Image Credit: Sino Biological US Inc

More antibodies for FcγRs (Partial)

Table 3. Source: Sino Biological US Inc

Cat# Antigen Species Clonality Ig Type Application
10389-MM22 CD16 Human Mouse MAb Mouse IgG1 FCM
10389-R221 CD16 Human Rabbit MAb Rabbit IgG FCM
50326-R015 CD16 Mouse Rabbit MAb Rabbit IgG WB, ELISA, IP
50326-RP02 CD16 Mouse Rabbit PAb Rabbit IgG ELISA
90013-RP02 CD16 Rhesus Rabbit PAb Rabbit IgG ELISA, IHC-P
10389-MM02 CD16a Human Mouse MAb Mouse IgG1 ELISA
10389-MM04 CD16a Human Mouse MAb Mouse IgG1 IHC-P
10389-MM41-F
    3 Citations
CD16a Human Mouse MAb Mouse IgG1 FCM
80017-R041 CD16a Rat Rabbit MAb Rabbit IgG ELISA
80017-RP02 CD16a Rat Rabbit PAb Rabbit IgG WB, ELISA, IP
11046-MM01 CD16b Human Mouse MAb Mouse IgG2a ELISA
11046-MM07 CD16b Human Mouse MAb Mouse IgG2a IHC-P
11046-RP01 CD16b Human Rabbit PAb Rabbit IgG ELISA
10374-MM01
    2 Citations
CD32a Human Mouse MAb Mouse IgG1 ELISA, FCM
10374-R020 CD32a Human Rabbit MAb Rabbit IgG WB
10374-T48 CD32a Human Rabbit PAb Rabbit IgG WB, ELISA
90015-MM06 CD32a Cynomolgus Mouse MAb Mouse IgG1 ELISA
90014-R046 CD32a Cynomolgus Rabbit MAb Rabbit IgG ELISA, FCM
10259-MM02 CD32b Human Mouse MAb Mouse IgG1 ELISA
10259-R112 CD32b Human Rabbit MAb Rabbit IgG ELISA, ELISA(Det)
10259-RP03
    2 Citations
CD32b Human Rabbit PAb Rabbit IgG ELISA
90014-MM06 CD32b Cynomolgus Mouse MAb Mouse IgG1 IHC-P
90014-R046 CD32b Cynomolgus Rabbit MAb Rabbit IgG ELISA, FCM
90014-RP02 CD32b Cynomolgus Rabbit PAb Rabbit IgG ELISA, IHC-P
10256-MM06 CD64 Human Mouse MAb Mouse IgG1 ELISA
10256-R407 CD64 Human Rabbit MAb Rabbit IgG ELISA, FCM
10256-RP02 CD64 Human Rabbit PAb Rabbit IgG WB, ELISA
10256-T16 CD64 Human Rabbit PAb Rabbit IgG ELISA
50086-R001 CD64 Mouse Rabbit MAb Rabbit IgG ELISA, IHC-P