Democratize cryo-EM with the Alpine Direct Detection Camera

The Alpine® direct detection camera from Gatan is considered to be the world’s first direct detection camera to democratize cryo-EM.

Advantages

Alpine is the world’s first direct detection camera to democratize cryo-electron microscopy (cryo-EM) imaging. Alpine enables the capture of high-resolution structures using 100 – 200 keV microscopes, making routine cryo-EM research accessible on cost-effective, commonly-used instruments and broadening its applicability within the wider research community.

  • Achieves a Detective Quantum Efficiency (DQE) that outperforms scintillator-based cameras by up to 2.4 times
  • Consistently acquires high-resolution data within the 100 – 200 keV range, effectively transforming screening microscopes into data collection tools
  • Enables 2D classification of small molecules and enhances macromolecule reconstructions
  • Enhances workflow efficiency by early failure detection, leading to quicker, improved structure generation
  • Real-time counting guarantees no trade-off between data collection speed and quality

Transthyretin resolved to 4.6 Å at 100 keV.

Transthyretin resolved to 4.6 Å at 100 keV. Transthyretin (55 kDa) resolved to 4.6 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) using CDS at ~5.2 e-/pixel/s. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Throughput versus total dose comparison.

Throughput versus total dose comparison. When combined with Latitude software, Alpine delivers high-quality data at the highest imaging throughputs from 100 – 200 keV. Image Credit: Gatan, Inc.

Aldolase resolved to 3.07 Å at 100 keV.

Aldolase resolved to 3.07 Å at 100 keV.  Aldolase (100 kDa) resolved to 3.07 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Apoferritin resolved to 2.7 Å at 100 keV.

Apoferritin resolved to 2.7 Å at 100 keV. Apoferritin (550 kDa) resolved to 2.7 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.