Growth factors for lung and digestive system organoids

It is challenging to produce robust organoids of the gastrointestinal and respiratory systems that accurately reflect the complex organ environment. High-quality growth factors (GFs) are crucial in this process, including EGF, FGF, Noggin, BMP, and RSPO1.

The development of bronchioalveolar and alveolar organoids is cooperatively induced by FGF10, HGF, and KGF/FGF7. FGF2 and IGF-1 also improve intestinal organoid plating.

Sino Biological offers a broad range of products for these growth factors and their corresponding receptors and activators, including c-MET, HGFAC, FGFR1, FGFR2, and BMPR2. These products support research and development of organoid cultures and various diseases associated with these molecules.

Featured growth factor proteins

Studies have demonstrated that FGF10, HGF, and KGF/FGF7 can promote the development of bronchioalveolar and alveolar organoids. To aid in studying organoid culture, Sino Biological has created high-quality FGF10, HGF, and FGF7. Advantages include:

  • High purity
  • HPLC verified
  • Actively validated
  • High lot-to-lot consistency
  • Low endotoxin
  • Cited by researchers

Human FGF10 protein Cat#: 10573-HNAE

Growth factors for lung and digestive system organoids

Purity: ≥95 % as determined by SDS-PAGE & SEC-HPLC. Image Credit: Sino Biological Inc.

Growth factors for lung and digestive system organoids

Cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. Image Credit: Sino Biological Inc.

Human HGF protein cat#: 10463-HNAS

Growth factors for lung and digestive system organoids

Purity: ≥95 % as determined by SDS-PAGE & SEC-HPLC. Image Credit: Sino Biological Inc.

Growth factors for lung and digestive system organoids

Ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. Image Credit: Sino Biological Inc.

Human KGF/FGF-7 protein cat#: 10210-H07E

Growth factors for lung and digestive system organoids

Purity: ≥95 % as determined by SDS-PAGE & SEC-HPLC. Image Credit: Sino Biological Inc.

Growth factors for lung and digestive system organoids

Cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. Image Credit: Sino Biological Inc.

More growth factor proteins (partial)

Sino Biological has created a collection of recombinant GFs and associated protein reagents to support the organoid culture of the gastrointestinal and respiratory systems and the investigation of numerous diseases connected to these elements.

Source: Sino Biological Inc.

Cat# GFs Species Expression Host Purity Activity Endotoxin
GMP-10605-HNAE EGF Human E. coli ≥95% Active <5 EU/mg
10605-H01H EGF Human HEK293 Cells >90% Active <1.0 EU/μg
10605-HNAE EGF Human E. coli ≥95% Active <5 EU/mg
50482-M01H EGF Mouse HEK293 Cells >95% Active <1.0 EU/μg
10014-HNAE FGF2 Human E. coli >95% Active <1.0 EU/μg
10210-H07E FGF7 Human E. coli ≥95% Active <0.01 EU/μg
10573-HNAE FGF10 Human E. coli ≥95% Active <5 EU/mg
10267-H02H Noggin Human HEK293 Cells >95% Active <1.0 EU/μg
10267-H08H Noggin Human HEK293 Cells >95% Active <1.0 EU/μg
10267-HNAH Noggin Human HEK293 Cells >95% Active <1.0 EU/μg
10426-H01H BMP2 Human, Mouse, Rat, Rhesus, Canine HEK293 Cells >95% Active <1.0 EU/μg
50439-M15H BMP4 Mouse HEK293 Cells >92%   <1.0 EU/μg
10214-H01H BMP5 Human HEK293 Cells >95%   <1.0 EU/μg
11083-H08H RSPO1 Human HEK293 Cells >95% Active <1.0 EU/μg
11083-H08H1 RSPO1 Human HEK293 Cells >98% Active <1.0 EU/μg
11083-HNAS RSPO1 Human CHO Stable Cells ≥95% Active <10 EU/mg
50316-M08S RSPO1 Mouse CHO Stable Cells >95% Active <1.0 EU/μg
10598-HNAE IGF1 Human E. coli >95% Active  
50437-MNAY IGF1 Mouse Yeast >95% Active  
10616-H08H FGFR1 Human HEK293 Cells >98% Active <1.0 EU/μg
16482-H02H FGFR1 Human HEK293 Cells >90% Active <1.0 EU/μg
50186-M02H FGFR1 Mouse HEK293 Cells >97% Active <1.0 EU/μg
90075-C02H FGFR1 Rhesus HEK293 Cells >95% Active <1.0 EU/μg
10824-H03H FGFR2 Human HEK293 Cells >90% Active <1.0 EU/μg
10824-H08H FGFR2 Human HEK293 Cells >97% Active <1.0 EU/μg
10824-H20B1 FGFR2 Human Baculovirus-Insect Cells >90% Active <1.0 EU/μg
16485-H02H FGFR2 Human HEK293 Cells >95% Active <1.0 EU/μg
16485-H08H FGFR2 Human HEK293 Cells >95% Active <1.0 EU/μg
51128-M02H FGFR2 Mouse HEK293 Cells >95% Active <1.0 EU/μg
10692-H08H c-MET Human HEK293 Cells >90% Active <1.0 EU/μg
10692-H20B1 c-MET Human Baculovirus-Insect Cells >90% Active <1.0 EU/μg
10692-H27H-B c-MET Human HEK293 Cells >95% Active <1.0 EU/μg
50622-M02H c-MET Mouse HEK293 Cells >92% Active <1.0 EU/μg
50622-M08H c-MET Mouse HEK293 Cells >90% Active <1.0 EU/μg
70008-D08H c-MET Canine HEK293 Cells >95% Active <1.0 EU/μg
80004-R02H c-MET Rat HEK293 Cells >95% Active <1.0 EU/μg
90304-C02H c-MET Cynomolgus, Rhesus HEK293 Cells >95% Active <1.0 EU/μg
90304-C08H c-MET Cynomolgus, Rhesus HEK293 Cells >95% Active <1.0 EU/μg
90304-CCCH c-MET Cynomolgus, Rhesus HEK293 Cells >90% Active <1.0 EU/μg
10329-H08H HGFAC Human HEK293 Cells >95%   <1.0 EU/μg
10551-H03H BMPR2 Human HEK293 Cells >90% Active <1.0 EU/μg

 

Featured antibodies for growth factor

Anti-FGF10 antibody, rabbit PAb cat#: 101637-T44

Growth factors for lung and digestive system organoids

Immunochemical staining of human FGF10 in human prostatic carcinoma tissue. Image Credit: Sino Biological Inc.

HGF neutralizing antibody cat# 10463-mh010

Growth factors for lung and digestive system organoids

Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF neutralizing Monoclonal Antibody. Image Credit: Sino Biological Inc.

Anti-c-MET antibody (APC), rabbit MAb cat#: 10692-R243-A

Growth factors for lung and digestive system organoids

Profile of anti-human C-Met (HGF R) reactivity on HepG2 cells analyzed by flow cytometry. Image Credit: Sino Biological Inc.