Host cell proteins: Detection and quantification

Host cell proteins (HCPs) are unwanted substances that can get into cell-based biotherapeutics, like recombinant proteins and antibodies during production. Chinese Hamster Ovarian (CHO) cells are commonly used to make these biotherapeutic products.

CHO cells naturally produce certain types of HCPs like PLBL2/PLBD2, PLA2G15, CLU, and LPL, which can harm the quality, effectiveness, and safety of biotherapeutics. Notably, HCPs can trigger unwanted immune reactions to the medicine. Because of this, there's a strong need for tests and substances to find and measure HCPs.

PLBL2/PLBD2 is a stubborn Host Cell Protein (HCP) present in CHO cells. Sino Biological, a world-renowned expert in recombinant technology, has recently created a special PLBL2/PLBD2 ELISA kit. This kit can precisely measure the amount of PLBL2/PLBD2 in Chinese hamster cells.

Sino Biological has also successfully produced antibodies and highly pure recombinant HCPs, which include PLBL2/PLBD2, PLA2G15, CLU, and LPL. These products make it easier to ensure the quality of biotherapeutics.

Host cell proteins: Detection and quantification

Sandwich ELISA for Detecting Host Cell Proteins. Image Credit: Sino Biological Inc.

Featured antibodies and ELISA kit for the detection of HCPs

PLBL2/PLBD2 ELISA kit, Chinese hamster: (Cat#: KIT5A7664)

Host cell proteins: Detection and quantification

Standard curve for PLBD2 quantification. Image Credit: Sino Biological Inc.

  • Assay range: 390.63–25000 pg/mL
  • Sensitivity: 60 pg/mL

Anti-PLBL2/PLBD2 antibody, Rabbit pAb: (Cat#: 13947-T24)

Host cell proteins: Detection and quantification

Immunochemical staining of human PLBL2/PLBD2 in human heart. Image Credit: Sino Biological Inc.

  • Applications: ELISA, IHC-P

Anti-Clusterin antibody, Mouse mAb: (Cat#: 11297-MM01)

Host cell proteins: Detection and quantification

Immunofluorescence staining of human CLU in HeLa cells. Image Credit: Sino Biological Inc.

Host cell proteins: Detection and quantification

HeLa whole cell lysate probed with anti-CLU mouse mAb. Image Credit: Sino Biological Inc.

  • Applications: ELISA, WB, FCM, ICC/IF

Featured recombinant HCPs

Recombinant PLBL2/PLBD2 protein, Chinese hamster: (Cat#: 5A7664-A08C) HPLC-verified

Host cell proteins: Detection and quantification

Image Credit: Sino Biological Inc.

  • Purity: ≥ 95%

Recombinant LPL protein, Chinese hamster: (Cat#: 5A7668-A08E)

Host cell proteins: Detection and quantification

Image Credit: Sino Biological Inc.

  • Purity: > 85%

More recommended products for HCPs (Partial list)

Source: Sino Biological Inc.

Product Type Cat# Product Name
Recombinant Protein 5A7663-A08C Chinese hamster PLA2G15 Protein (His Tag)
Recombinant Protein 5A7667-A08C Chinese hamster Clusterin Protein (His Tag)
Antibody 5A7664-MM23 Anti-PLBL2 / PLBD2 Antibody
Antibody 5A7668-T16 Anti-LPL Antibody, Rabbit pAb
Antibody 11297-RP01 Anti-Clusterin Antibody, Rabbit pAb
Antibody 11297-R210 Anti-Clusterin Antibody, Rabbit mAb

 

More antibodies against HCPs