Induced pluripotent stem cell (iPSC) cytokines

Induced pluripotent stem cells (iPSCs) are a hopeful alternative for analyzing antigen expression in vitro and developing novel cellular therapies. iPSCs have been derived from differentiated adult somatic cells that are reprogrammed into an embryonic-like pluripotent state by making use of various transcription factors. They are capable of distinguishing into any kind of cell lineage.

With their limitless capacity for self-renewal and differentiation, such iPSCs hold enormous abilities in regenerative medicine, drug testing, disease modeling, and innovative cell or gene therapies.

The extensive selection of cytokines and growth factors, such as GM-CSF, DLL4, Activin A, NOG, VEGF, TNF-α, IL-2, FGF, IL1β, and EGF, are necessary components for distinguishing human iPSCs into several cell lineages. The applications of cytokines in iPSC studies are displayed in Table 1.

Table 1. List of cytokines and the applications in iPSC research. Source: Sino Biological Inc.

Essential cytokines in iPSC research Application in research
GM-CSF, IL-3, M-CSF iPSC-derived monocytes and macrophages
DLL-4, IL-2, BMP4, TGF-β, IL33, IL10 iPSC-derived regulatory T cells (Tregs)
Activin A, BMP-4, MRAP, FGF2 iPSC-derived nephron progenitors
NOG, TNF-α, BMP-2, TGF-β1, FGF2 iPSC-derived mesenchymal stem cells (MSCs)
FGF10, BMP-4, TGF-β, FGF2 iPSC-derived lung epithelial cell lineages
VEGF121, FGF2, Ang-1, TNF-α iPSC-derived endothelial cells
EGF, OSM, HGF iPSC-derived hepatocytes
IL-1ββ, TPO, SCF iPSC-derived megakaryocytes

 

Being a leading global supplier of high-quality recombinant cytokines, Sino Biological aids in quickly developing stem cell research.

Induced pluripotent stem cell (iPSC) cytokines

Figure 1. Differentiation of induced pluripotent stem cells (iPSCs). Image Credit: Sino Biological Inc.

Featured recombinant iPSC cytokines

Sino Biological provides an extensive collection of stem cell-related growth factors and related protein reagents to iPSC research.

  • High purity
  • Activity-validated
  • Cited by researchers
  • HPLC-verified

Human DLL4 protein (Cat#: 10171-H02H) 9 citations

Induced pluripotent stem cell (iPSC) cytokines

Immobilized mouse NOTCH1 (Cat: 50654-M08B) can bind human DLL4. Image Credit: Sino Biological Inc.

Human GM-CSF protein (Cat#: 10015-HNAH) 2 citations

Induced pluripotent stem cell (iPSC) cytokines

Cell proliferation assay using TF-1 human erythroleukemic cells. Image Credit: Sino Biological Inc.

Human Activin A protein (Cat#: 10429-HNAH) 5 citations

Induced pluripotent stem cell (iPSC) cytokines

Ability to inhibit proliferation of MPC-11 cells. Image Credit: Sino Biological Inc.

Human FGF10 protein (Cat#: 10573-HNAE) 3 citations

Induced pluripotent stem cell (iPSC) cytokines

Cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. Image Credit: Sino Biological Inc.

Mouse NOG protein (Cat#: 50688-M02H) 6 citations

Induced pluripotent stem cell (iPSC) cytokines

Ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. Image Credit: Sino Biological Inc.

Human EGF protein (Cat#: 10605-HNAE) 57 citations

 Induced pluripotent stem cell (iPSC) cytokines

Cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. Image Credit: Sino Biological Inc.

More recombinant iPSC cytokines (Partial)

Table 2. Source: Sino Biological Inc.

Cat# Species Proteins Expression Host Purity Activity Tag
11792-H08H
6 Citations
Human M-CSF/CSF1 HEK293 Cells > 95 % Active His
51112-MNAH
11 Citations
Mouse M-CSF/CSF1 HEK293 Cells > 95 % Active Native
10015-H01H
4 Citations
Human GM-CSF/CSF2 HEK293 Cells > 97% Active hFc
51048-MNAH
5 Citations
Mouse GM-CSF/CSF2 HEK293 Cells > 95 % Active Native
80020-R01H Rat GM-CSF/CSF2 HEK293 Cells > 95 % Active hFc
50659-M08H Mouse Activin A HEK293 Cells > 88 % Active His
10267-H02H
3 Citations
Human NOG/Noggin HEK293 Cells > 95 % Active hFc
10267-HNAH Human NOG/Noggin HEK293 Cells > 95 % Active Native
10171-HCCH
9 Citations
Human DLL4 HEK293 Cells ≥ 90 %
(SEC-HPLC)
Active Native
50640-M08H
1 Citation
Mouse DLL4 HEK293 Cells > 96 % Active His
90882-C08H
3 Citations
Rhesus DLL4 HEK293 Cells > 95 % Active His
10602-HNAE
30 Citations
Human TNF-α E. coli ≥ 95 %
(SEC-HPLC)
Active Native
50349-MNAE
15 Citations
Mouse TNF-α E. coli > 98 % Active Native
60002-FNAE Ferret TNF-α E. coli > 95 % Active Native
70003-DNAE
1 Citation
Canine TNF-α E. coli > 96 % Active Native
80045-RNAE
6 Citations
Rat TNF-α E. coli > 93 % Active Native
90330-CNAE-B Cynomolgus TNF-α E. coli > 95 % Active Native
10804-H08H
16 Citations
Human TGF-β HEK293 Cells > 95 % Active His
50698-M08H
6 Citations
Mouse TGF-β HEK293 Cells > 95 %   His
80116-R08H
4 Citations
Rat TGF-β HEK293 Cells > 90 %   His
90294-C08H
5 Citations
Cynomolgus TGF-β HEK293 Cells > 95 % Active His
10139-HNAE
13 Citations
Human IL-1β E. coli ≥ 95 %
(SEC-HPLC)
Active Native
50101-MNAE
14 Citations
Mouse IL-1β E. coli > 97 % Active Native
70018-DNAE Canine IL-1β E. coli > 96 % Active Native
80023-RNAE
4 Citations
Rat IL-1β E. coli > 98 % Active Native
11848-H08H
2 Citations
Human IL2 HEK293 Cells > 95 % Active His
11848-HNAH1-E
1 Citation
Human IL2 HEK293 Cells > 95 %
(SEC-HPLC)
Active Native
11848-HNAE2 Human IL2 E. coli ≥ 95 %
(SEC-HPLC)
Active Native
51061-MNAE
8 Citations
Mouse IL2 E. coli > 92 % Active Native
70014-DNAE
2 Citations
Canine IL2 E. coli > 95 % Active Native
11858-HNAE
1 Citation
Human IL3 E. coli > 95 % Active Native
51066-MNAH
1 Citation
Mouse IL3 HEK293 Cells > 95 % Active Native
80235-R08B
1 Citation
Rat IL3 Baculovirus-Insect Cells > 95 % Active His
10947-H07H
6 Citations
Human IL10 HEK293 Cells > 85 % Active His
10947-HNAE Human IL10 E. coli > 95 % Active Native
50245-MNAE
7 Citations
Mouse IL10 E. coli > 95 % Active Native
10368-HNAE
11 Citations
Human IL33 E. coli > 95 % Active Native
70005-DNAE
5 Citations
Canine IL33 E. coli > 97 % Active Native
10426-HNAE
10 Citations
Human, Mouse, Rat, Rhesus, Canine BMP2 E. coli > 95 % Active Native
63003-ZNAE
3 Citations
Danio rerio BMP2 E. coli > 95 % Active Native
50439-M15H
7 Citations
Mouse BMP4 HEK293 Cells > 92 %   rFc
10605-H01H
40 Citations
Human EGF HEK293 Cells > 90 % Active hFc
10605-HNAE
57 Citations
Human EGF E. coli ≥ 95 %
(SEC-HPLC)
Active Native
50482-M01H
3 Citations
Mouse EGF HEK293 Cells > 95 % Active hFc
70013-D07E
1 Citation
Canine EGF E. coli > 98 % Active His
80446-RNAE
2 Citations
Rat EGF E. coli > 95 % Active Native
10014-HNAE
44 Citations
Human FGF2 E. coli > 95 % Active Native
50037-M07E
5 Citations
Mouse FGF2 E. coli > 95 % Active His
10573-HNAE
3 Citations
Human FGF10 E. coli > 95 % Active Native
10463-HNAS
16 Citations
Human HGF CHO Stable Cells ≥ 95 %
(SEC-HPLC)
Active Native
50038-MNAH
3 Citations
Mouse HGF HEK293 Cells > 95 % Active Native
70063-DNAH Canine HGF HEK293 Cells > 95 % Active Native
80429-RNAH
2 Citations
Rat HGF HEK293 Cells > 85 % Active Native
90286-CNAH Cynomolgus HGF HEK293 Cells > 95 % Active Native
11066-HNAH
8 Citations
Human, Cynomolgus VEGF165 HEK293 Cells > 90 %
(SEC-HPLC)
Active Native
11066-H27H-B
13 Citations
Human VEGF165 HEK293 Cells > 95 % Active His & AVI
63004-ZNAB
7 Citations
Danio rerio VEGF165 Baculovirus-Insect Cells > 90 % Active Native
10008-HNAH
1 Citation
Human VEGF121 HEK293 Cells > 95 %
(SEC-HPLC)
Active Native
10009-HNAB
2 Citations
Human VEGF183 Baculovirus-Insect Cells > 95 % Active Native
29714-HNAH Human VEGF110 HEK293 Cells > 95 % Active Native
50159-MNAB
7 Citations
Mouse VEGF164 Baculovirus-Insect Cells > 90 % Active Native
80006-RNAB
2 Citations
Rat VEGF164 Baculovirus-Insect Cells > 86 % Active Native
13667-H02H1 Human Angiopoietin-1 HEK293 Cells > 85 %   hFc
50300-M07H Mouse Angiopoietin-1 HEK293 Cells > 95 %   His
90025-C07H Cynomolgus Angiopoietin-1 HEK293 Cells > 95 %   His
15444-H01H Human MRAP HEK293 Cells > 90 %   hFc
10452-HNAH
2 Citations
Human OSM HEK293 Cells > 95 % Active Native
50112-M08H
3 Citations
Mouse OSM HEK293 Cells > 95 %   His
10451-H08B
2 Citations
Human SCF Baculovirus-Insect Cells > 92 % Active His
50487-M08B Mouse SCF Baculovirus-Insect Cells > 95 % Active His
10381-H07H Human Thrombopoietin HEK293 Cells > 90 % Active His
10381-H08C
1 Citation
Human Thrombopoietin CHO Stable Cells > 85 % Active His
50146-M08H Mouse Thrombopoietin HEK293 Cells > 90 % Active His

 

Featured antibodies for stem cell research

High-quality antibodies are crucial in the characterization of iPSC cell differentiation. Sino Biological offers high-quality antibodies for stem cell markers and various lineage markers with applications in ELISA, IHC-IP, FCM, and IF or ICC.

Differentiated cell type: Monocytes and macrophages

Anti-CD45 antibody (Cat#: 102001-T08) 1 citation

Type: Rabbit PAb
Ig Type: Rabbit IgG
Applications: IHC-P

Induced pluripotent stem cell (iPSC) cytokines

Immunochemical staining of mouse Ptprc in mouse brain. Image Credit: Sino Biological Inc.

Anti-CD14 antibody (Cat#: 10073-R002) 1 citation

Type: Rabbit Mab
Ig Type: Rabbit IgG
Applications: FCM

Induced pluripotent stem cell (iPSC) cytokines

Flow cytometric analysis of CD14 expression on human whole blood monocytes. Image Credit: Sino Biological Inc.

Differentiated cell type: Nephron progenitors

Anti-E-cadherin antibody (Cat#: 10204-MM04) 7 citations

Type: Mouse Mab
Ig Type: Mouse IgG1
Applications: ELISA, ICC/IF

Induced pluripotent stem cell (iPSC) cytokines

Immunofluorescence staining of human E-cadherin in MCF-7 cells. Image Credit: Sino Biological Inc.

Anti-CD31 antibody (Cat#: 10148-MM13) 1 citation

Type: Mouse Mab
Ig Type: Mouse IgG1
Applications: FCM, ICC/IF

Induced pluripotent stem cell (iPSC) cytokines

Immunofluorescence staining of Human CD31 in HUVEC cells. Image Credit: Sino Biological Inc.

Differentiated cell type: Regulatory T cells

Anti-CD4 antibody (Cat#: 60003-MM02-A) 3 citations

Type: Mouse Mab
Ig Type: Mouse IgG1
Applications: FCM

Induced pluripotent stem cell (iPSC) cytokines

Analysis of CD4 expression on spleen lymphocytes. Image Credit: Sino Biological Inc.

Anti-CD25/IL2R alpha antibody (Cat#: 50292-R049-C) 1 citation

Type: Rabbit Mab
Ig Type: Rabbit IgG
Applications: FCM

Induced pluripotent stem cell (iPSC) cytokines

Flow cytometric analysis of mouse IL2RA(CD25) expression on mouse splenocytes. Image Credit: Sino Biological Inc.

Other related molecules