Sierra SPR-32 Pro for complex high-throughput applications

Sierra SPR-32 Pro offers high-throughput SPR analysis of molecular interactions ranging from initial screening to elaborate kinetic characterization.

The high throughput offers a collection of eight channels/four spots facilitating 13,200 interactions in 24 hours.

Highlights

Surface plasmon resonance (SPR)

Tailored for complex high-throughput applications

High throughput

Over 4400 samples per day producing beyond 13,200 control subtracted binding responses for applications like epitope characterization and antibody, fragment, or small molecule screening.

Control and sensitivity

In-channel control splits the number of assay cycles needed and enhances data quality by avoiding possible changes among various sample preparations.

Flexible

Select any combination between 1 and 8 needles for flexibility, optimum performance, and various applications.

Ease of use

Sierra SPR control software integrates the flexibility of the hardware with a user-friendly drag-and-drop method editor. 

Features

Combining industry-leading performance with high-throughput SPR analysis

New possibilities in real-time, label-free detection

Key system features allow flexible instrument functioning and strong assay development while upholding system performance, detection sensitivity, and throughput.

  • 32 individually addressable detection spots with eight flow cells with four spots to examine more controls and targets per injection
  • Continuous flow hydrodynamic isolation microfluidics allow rapid transitions among solutions for precision kinetics in a range of sample matrices
  • Individual needle control has the potential to control the process from one to eight samples per injection
  • Flexible buffer configuration saves samples and time
  • Easy-to-use software provides high-throughput SPR data analysis and collection

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

Breakthrough SPR detection for sensitivity

Using a high-speed optical scanning and high-intensity laser light source, the SPR+ detector gets its sensitivity by integrating imaging surface plasmon resonance (SPRi). This arrangement helps highly sensitive imaging of comparatively huge two-dimensional sensor arrays, whereas the intensity of the light source allows the application of a high-speed camera, which consequently gathers more resonance measurements per scan.

The net result is a signal-to-noise ratio of 0.02 RU (RMS) and increased precision while quantifying small response variations, often observed for small or fragment molecule binding experiments.

Automated high-throughput sample analysis

Multiple user-defined configurations are feasible to enhance high-throughput SPR analysis using 32 detection spots available during each analysis cycle. This can comprise 31 control-subtracted injections, a maximum of four targets, or three in-line controls per channel.

The autosampler is compatible with a broad range of 96- and 384-well microtiter plates. The dual-plate sample deck with a common rack position enables improved automation.

The instrument can work in high-throughput mode and examine over 4400 samples daily when fitted with an optional plate handling robot. This leads to over 13,200 control-subtracted interactions.

Sierra SPR®-32 Pro could be effortlessly combined into an automated scheduling software through API.

Sierra SPR-32 Pro for complex high-throughput applications

High-throughput SPR Analysis with 31 targets and 1 control surface. Image Credit: Bruker Life Sciences Mass Spectrometry

Frame inject feature for multi-buffer analysis

The eight-syringe continuous flow buffer is a robust assay optimization and development instrument. It aids high-throughput SPR analysis within an array of conditions, which include various salt, pH, solvent or detergent concentration, etc.

Concurrent multi-buffer analysis saves reagents and time while enhancing information throughput.

Benefits

High throughput with performance

The Sierra SPR®-32 Pro system is a powerful instrument for high-throughput applications, such as epitope characterization and antibody, fragment, or small molecule screening.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

Concurrent injection of a maximum of eight samples enables high-throughput assay development and optimization, along with quick quantitative analysis of purified and crude samples.

Hydrodynamic isolation (HI) continuous flow microfluidic technology addresses samples as flowing streams onto the sensor array. This enables treating eight samples on a standalone system, which could be increased to more than 4400 samples per day via optional plate handling robotics.

Flexibility optimizes productivity

Individual needle control (INC) enhances the flexibility, scope, and performance of applications, simultaneously saving materials and time when throughput is not the critical necessity.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

A maximum of eight buffers can be employed simultaneously in the instrument, with frame inject. The capacity to evaluate varied ionic strength, pHs, solvent or detergent concentration, and inhibitors or co-factors' effect in a single assay leads to simpler, enhanced assays that eventually improve productivity and throughput.

The four-sensor per channel design offers the utmost flexibility for control analysis while maintaining throughput for the most complicated analyses.

Assay control and sensitivity 

Four independently addressable detection spots are configured in each of the eight channels of the Sierra SPR®-32 Pro system. Samples and the active surface can be assessed concurrently on a maximum of three control surfaces. In several SPR applications, early identification of nonspecific binding to regulated proteins is crucial.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

In small molecule characterization and screening, binding to serum proteins, such as BSA and HSA, is a main part of drug development. The extra in-channel control splits the number of assay cycles needed and enhances data quality by avoiding potential changes among several sample preparations.

The SPR+ detector integrates imaging SPR (SPRi) with high-speed optical scanning and a high-intensity laser light source. The outcome is enhanced signal-to-noise and increases precision while quantifying small response variations.

Ease of use

The Sierra SPR control software offers the ideal solution for all users—from initial assay development to pre-defined method templates. It integrates the flexibility of the hardware with a user-friendly drag-and-drop method editor. Personal workflow structuring is supported by a well-defined plate and method library, and the data view enables real-time visual monitoring of flow cells.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

Consumables

Component

  • High-capacity amine sensor

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

  • Amine Coupling Kits

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

  • Microtiter Plates

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry 

Applications of surface plasmon resonance

Tackling challenging targets with a high-throughput biophysical screening at Novartis

In this real-time example, automation, automated data analysis, and multiplexing using Genedata Screener take SPR further ahead.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

How to influence ligand density using amine coupling

Identifying the perfect immobilization level to achieve optimal kinetic measurements is crucial. Here, the influencing parameters are analyzed on various example targets.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

Screening and characterization of small molecule binding to protein targets

Real-time, label-free (RT-LF) analysis by SPR detection is a robust tool for the biophysical definition of small molecule drugs and drug candidates.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

Screening and characterization of biotherapeutics

Real-time, label-free (RT-LF) analysis by SPR detection is a robust tool for the biophysical definition of protein therapeutics.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry

Investigation of condition-dependent binding using frame inject

The conventional examination of condition-dependent binding needs high reagent costs along with a lengthy run time. Frame inject saves cost and time at low sample consumption.

Sierra SPR-32 Pro for complex high-throughput applications

Image Credit: Bruker Life Sciences Mass Spectrometry