TurboCHO system for recombinant antibody expression in mammalian cells

Expressing recombinant antibodies in mammalian cells is essential when post-translational alterations and proper folding are required for downstream applications. The most common problems of antibody expression projects are inadequate antibody yield, lengthy completion time, often requiring many months, and cost overruns.

To address these constraints, GenScript created its patented TurboCHOTM platform, which provides higher yield performance in less time. This low-cost production method enables small- to large-scale (microgram to kilogram) antibody production with great consistency, making it an ideal complement for target identification, pre-evaluation pipelines, and overall user value.

GenSmartTM Codon Optimization, an industry-standard free codon optimization tool, assists users in designing the gene sequence to enhance their chances of getting functional antibodies using the TurboCHOTM expression system.

Highlights

  • Increased yield
  • Multiple isotypes available
  • Verified consistency

Services

TurboCHOTM high throughput

Developed for high-throughput screening in as little as two weeks

TurboCHOTM express

Yields up to gram, from three weeks

TurboCHOTM high performance

Yield up to kg, from 6–7 weeks

Service specifications

From beginning to end, achieve success with seamless CHO expression systems.

Source: GenScript

  TurboCHO™ HT
(High Throughput)
TurboCHO™ Express TurboCHO™ HP
(High Performance)
Requested Protein Amount Up to 5 mg Up to 500 mg 500 mg to kilograms
Production Time Starting From Gene Synthesis 2 weeks 3 weeks 6 weeks
Deliverables Purity ≥90% Protein: Purity ≥90%
Antibody: Purity ≥95%
Con. ≥1.0 mg/mL
Endotoxin level: ≤1 EU/mg
Protein: Purity ≥90%
Antibody: Purity ≥95%
Con. ≥1.0 mg/mL
Endotoxin level: ≤1 EU/mg
Additional Services ELISA, Cell-based assay, FACS screening, Octet® BLI assay, Biacore™ SPR assay, etc.

 

Note:

This customized solution applies to all types of proteins, antibody species, isotypes, and fragments.
All incoming projects are subjected to evaluation.
Additional QC and characterizations are offered with an extra charge upon request.
Pilot expression evaluation can be requested for CHO-Express/HP.

Case studies

High expression titers of multiple isotypes in different TurboCHO™ platforms

TurboCHO system for recombinant antibody expression in mammalian cells

Image Credit: GenScript 

High purity of antibodies expressed in TurboCHO™ system

1. Bispecific antibody production, titer: 374 mg/L with purity >99%

TurboCHO system for recombinant antibody expression in mammalian cells

Image Credit: GenScript

2. scFv antibody production, titer: 118 mg/L with purity >99%

TurboCHO system for recombinant antibody expression in mammalian cells

Image Credit: GenScript

3. VHH-FC production, titer: 169 mg/L with purity >99%

TurboCHO system for recombinant antibody expression in mammalian cells

Image Credit: GenScript